Premature translation termination mediates triosephosphate isomerase mRNA degradation.
نویسندگان
چکیده
منابع مشابه
The nonsense-mediated mRNA decay pathway triggers degradation of most BRCA1 mRNAs bearing premature termination codons.
Germline mutations in the BRCA1 gene are scattered over the 22 coding exons and most of them generate premature termination codons (PTCs). A mechanism called nonsense-mediated mRNA decay (NMD) is known to specifically degrade transcripts with PTCs; however, steady-state amounts of mutant BRCA1 mRNAs have very rarely been measured. Although growing evidence implicates downstream exon-exon juncti...
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We have analyzed a cloned #{176}-thalassemia ($#{176}-thal) gene from a patient doubly heterozygous for hemoglobin Lepore and $#{176}-thalassemia. Studies of 3H-uridine incorporation into 9-globin mRNA in this patient’s erythroblasts suggested an intranuclear defect in both $ and Lepore (#{246} ) mRNA synthesis, as did S nuclease analysis of nuclear RNA. However, the nucleotide sequence of the ...
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Translational stop codons were introduced at various locations in the protein-coding regions of the monocistronic bla and ompA gene transcripts of Escherichia coli, and the decay characteristics of the upstream and downstream mRNA segments were analyzed. Premature termination of translation at codon position 26 reduced the stability of both the translated and ribosome-free segments of bla mRNA,...
متن کاملTranslation termination factor eRF3 mediates mRNA decay through the regulation of deadenylation.
Messenger RNA decay, which is a regulated process intimately linked to translation, begins with the deadenylation of the poly(A) tail at the 3' end. However, the precise mechanism triggering the first step of mRNA decay and its relationship to translation have not been elucidated. Here, we show that the translation termination factor eRF3 mediates mRNA deadenylation and decay in the yeast Sacch...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1988
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.8.2.802